Nitrification, the aerobic conversion of ammonia into nitrates, is one of the most important functions in an aquaponics system as it reduces the toxicity of the water for fish, and allows the resulting nitrate compounds to be removed by the plants for nourishment.[1] Ammonia is steadily released into the water through the excreta and gills of fish as a product of their metabolism, but must be filtered out of the water since higher concentrations of ammonia (commonly between 0.5 and 1 ppm)[citation needed] can kill fish. Although plants can absorb ammonia from the water to some degree, nitrates are assimilated more easily,[2] thereby efficiently reducing the toxicity of the water for fish.[1] Ammonia can be converted into other nitrogenous compounds through healthy populations of:
Nitrosomonas: bacteria that convert ammonia into nitrites, and
Nitrobacter: bacteria that convert nitrites into nitrates.
In an aquaponics system, the bacteria responsible for this process form a biofilm on all solid surfaces throughout the system that are in constant contact with the water. The submerged roots of the vegetables combined have a large surface area, so that many bacteria can accumulate there. Together with the saliency of ammonia and nitrites in the water, the surface area determines the speed with which nitrification takes place. Care for these bacterial colonies is important as to regulate the full assimilation of ammonia and nitrite. This is why most aquaponics systems include a biofiltering unit, which helps facilitate growth of these microorganisms. Typically, after a system has stabilized ammonia levels range from 0.25 to 2.0 ppm; nitrite levels range from 0.25 to 1 ppm, and nitrate levels range from 2 to 150 ppm.[citation needed] During system startup, spikes may occur in the levels of ammonia (up to 6.0 ppm) and nitrite (up to 15 ppm), with nitrate levels peaking later in the startup phase.[citation needed] Since the nitrification process acidifies the water, non-sodium bases such as potassium hydroxide or calcium hydroxide can be added for neutralizing the waters pH[1] if insufficient quantities are naturally present in the water to provide a buffer against acidification. In addition, selected minerals or nutrients such as iron can be added in addition to the fish
waste that serves as the main source of nutrients to plants.[1]
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